In QBO there is an entirely different section in your settings for Payroll. This is where you set up your Federal and State taxes, the payroll bank account you're using for tax payments and payroll checks, etc. If you do not update the banking information in these settings when you change your bank account, then QBO will continue to take the money from the account you established in there. Please note: You need to make sure that you have no outstanding tax payments or payroll checks before you update this account so be sure all transactions have cleared that account first before making any changes.  Also note that before you can begin using this new bank account that QB has to verify your new bank account which can take from 1-3 days. When you start using the new account and you run payroll, check the top of the screen to make sure you select the correct/new account the money is coming from.  Hope this helps someone out there!  Jennifer M. QuickBooks Online Payroll Expert/ProAdvisor