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When utilizing QuickBooks Desktop (QBDT), turning off the Don't allow any applications to access this company file feature will not affect your payroll updates. The option primarily pertains to third-party and Intuit-related applications.
By turning off this option, you are allowing specific applications to access your company file, including any necessary updates related to payroll. It ensures that you can continue to receive timely payroll updates, such as tax table changes, compliance modifications, and other crucial payroll-related information delivered through Intuit's servers. Let me guide you through the process.

Check out this article for more insights into this process: Block applications from accessing the QuickBooks company file.
Let me know if you have any clarifications or other concerns. I’ll be around to answer them for you. Have a great day ahead!