Welcome back to the Community, @tippykayak. We can check the template of your estimate to ensure it will print the subtotal. Follow along below to get this fix right away. 

We have to make sure that the Print option for Subtotal is enabled. Here's how:

1. In your QuickBooks Desktop (QBDT) company file, select the Lists menu.
2. Next, choose Templates. 
3. Then, select the template you use for the estimate.
4. Once opened, click the Additional Customization button.
5. Go to the Footer tab.
6. From there, ensure that the Print box for Subtotal is checked.
7. You can also make changes and select OK twice to confirm the action. 

After that, attempt to create and print the estimate and select the correct template. Check to see if this will also include the subtotal.

However, if the issue persists, you may want to perform several troubleshooting steps to resolve printing problems in QBDT. For detailed instructions, see this article: Resolve printing issues. 

Tracking estimates made for each of your customers is a breeze in QBDT. To do so, simply go to the Reports menu and scroll down to the Job, Time & Mileage section. Then, select the Estimates by Job report.

Let me know how this goes and feel free to leave a reply below. I also appreciate it if you can add updates about this. I'm always around here in the Community and ready to lend a hand. Have a great day ahead.