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Buy nowThanks for getting back to the thread, @alliejack.
As long as your bank is linked to QuickBooks Online, manually uploading the file will not prevent QuickBooks Online from downloading transactions automatically.
Furthermore, if you are not receiving the most recent transactions or updates appear to be stuck, please follow the workarounds listed below:
You can read this article for more detailed steps and information: What to do if you get a bank error or can't download transactions in QuickBooks Online.
If you need to exclude bank transactions, you can refer to this article: Exclude a bank transaction you downloaded into QuickBooks Online.
Kindly get back to the thread if you have further concerns with your bank transactions. We're available 24/7. Have a great day.